In the short lived "Spider-Man: The New Animated Series," which aired on MTV for one season of thirteen episodes in 2003, Neil Patrick Harris voiced Peter Parker.
The show, which loosely continued the story of the 2002 Spider-Man movie, was made using CGI with cel shading and was produced by Mainframe Entertainment for Sony Pictures Television.
The show took place at some point after the first film, because Norman Osborn (the Green Goblin) is dead, and Peter Parker is in college. It focused mainly on Peter trying to use his powers for good and struggling with balancing his personal life with his superhero life, and revolved around Voltaire's idea of "with great power comes great responsibility."
The series was well received by fans, but less so by critics. Critics thought of the cel shaded animation style and the more violent interpretation of Spider-Man favorably, but didn't like the time line of the film and how loosely it was based on the comics.
The last episode created some confusion because it depicted Peter giving up being Spider-Man for good, even though a second Spider-Man film had just been announced. This meant that the show was then an alternative time line, and was unrelated to the films.